Journal: iScience

Article Title: Atractylodin modulates ASAH3L to improve galactose metabolism and inflammation to alleviate acute lung injury

doi: 10.1016/j.isci.2024.110751

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Article Snippet: The kits for TNF-α (E-EL-H0109c), IL-1β (E-EL-H0149c), IL-6 (E-EL-H6156), and MCP-1 (E-EL-H6005) in human species were purchased from the Elabscience Biotechnology Co, Ltd. (Wuhan, China).

Techniques: Recombinant, Transfection, CCK-8 Assay, Enzyme-linked Immunosorbent Assay, Picogreen Assay

Journal: Journal of Cellular and Molecular Medicine

Article Title: C1q/ TNF ‐related protein 9 inhibits the cholesterol‐induced Vascular smooth muscle cell phenotype switch and cell dysfunction by activating AMP‐dependent kinase

doi: 10.1111/jcmm.13196

Figure Lengend Snippet: CTRP 9 treatment alleviates cholesterol loading‐induced VSMC pro‐inflammatory factor secretion ( A ) Cell viability of VSMC s, measured using CCK 8, after incubation with CTRP 9 for 72 hrs. ( B ‐ D ) VSMC s were treated for 72 hrs with different concentrations of CTRP 9 (0–10 μg/ml) in the presence of 5 μg/ml cholesterol or were left untreated (control) before measurement of ( B ) IL ‐6 secretion, ( C ) IL ‐1β secretion and ( D ) MCP ‐1 secretion. Values represent the means ± S.D. of triplicate reactions and are representative of three independent experiments. ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: Recombinant human CTRP9 (6537‐TN‐050) and all ELISA‐based detection systems (IL‐6, VAL102, IL‐1β, VAL101 and MCP‐1, DCP00) were purchased from R&D Systems (Minneapolis, MN, USA).

Techniques: CCK-8 Assay, Incubation

Journal: STAR Protocols

Article Title: Super-STORM: Molecular Modeling to Achieve Single-molecule Localization with STORM Microscopy

doi: 10.1016/j.xpro.2019.100012

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Article Snippet: Recombinant human CCL2 , Biolegend , Cat# 571402.

Techniques: Recombinant, Blocking Assay, Antibody Labeling, Electron Microscopy, Software

Journal: Molecular Cell

Article Title: Cross-Regulation between TDP-43 and Paraspeckles Promotes Pluripotency-Differentiation Transition

doi: 10.1016/j.molcel.2019.03.041

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Article Snippet: HeLa , ATCC: CCL-2 , RRID: CVCL_0045.

Techniques: Recombinant, Produced, Gentle, Knock-Out, Reverse Transcription, TaqMan Assay, Labeling, Staining, Flow Cytometry, Western Blot, Clarification Assay, Membrane, Sample Prep, Sequencing, Derivative Assay, Next-Generation Sequencing, Gene Expression, Fractionation, Activation Assay, Nucleic Acid Electrophoresis, CRISPR, Plasmid Preparation, Modification, shRNA, Software